imagej color threshold black pixels still selecting

For both kinds, depth-cueing is turned off when set to zero (i.e.100% of intensity in back to 100% of intensity in front) and is on when set at 0 < n 100 (i.e.( 100 − n)% of intensity in back to 100% intensity in front). Hi, I am trying to analyze fluorescence intensity in one colour images with Fiji. Interior Depth-Cueing works only on brightest-point projections. Surface Depth-Cueing works only on nearest-point projections and the nearest-point component of other projections with opacity turned on.

Two kinds of depth-cueing are available: Surface Depth-Cueing and Interior Depth-Cueing. Select a range of 1-255 for the resulting image Do particle analysis as in 3. The trade-off for this increased realism is that data points shown in a depth-cued image no longer possess accurate densitometric values. o What did AND operation do when in the other image, pixel intensity values are either 0 or 255 (binary), and in the other image, pixel intensity values can vary from 0 to 255 (8-bit image) Set threshold via Image - Adjust Threshold. The depth-cueing parameters determine whether projected points originating near the viewer appear brighter, while points further away are dimmed linearly with distance. Surface/Interior Depth-Cueing Depth cues can contribute to the three-dimensional quality of projection images by giving perspective to projected structures.

Imagej color threshold black pixels still selecting